Abstract
The activin type II receptor belongs to the TGF-ß receptor family and plays a role in mediating signals from transforming growth factor-beta (TGF-ß) ligands, including superfamily members like myostatin, which negatively regulate skeletal muscle growth. TGF-ß ligands exert diverse biological effects on cell differentiation and growth. In mammals, five distinct receptor types bind TGF-ß family ligands, with activins specifically interacting with ActRIIA and ActRIIB receptors. These receptors also bind other TGF-ß family proteins. In fish, their primary role is associated with muscle growth. For this study on the activin type IIB receptor (ActRIIB), I selected Labeorohita (commonly known as rohu), an economically important carp species widely consumed in Asian countries. Blood samples were collected from rohu specimens obtained from the Government Fisheries Department in Vehari and the Sutlej River. Following sample collection, PCR was performed to isolate the target gene. In addition to PCR-based gene isolation, I conducted phylogenetic analyses to investigate the evolutionary history of these genes at both sequence and structural levels. This research enhances our understanding of the functional and evolutionary aspects of activin genes in fish, providing a foundation for future studies in this field.
Replies